How to dissolve Chitosan?
Chitosan is positively charged and soluble in weak acidic solutions. Chitosan powder is soluble in glycolic, acetic or lactic acid, amongst others. The solubility is dependent on the concentration and molecular weight which determines the viscosity while being little influenced by the degree of deacetylation. To increase the solubility, the solution should be heated and stirred. ChitopharmTM forms clear solutions that can be directly incorporated in your pharmaceutical production.
How to prepare Chitosan Hydrogel?
Chitosan forms gels when dispersed in a weak acid. Low (LMWC), medium (MMWC) or high (HMWC) molecular weight chitosan are usually dispersed in 2.5 % (v/w) acetic acid solution. Alternatively, 0.25, 1, 1.5, 2 and 4% acetic acid solutions can be used depending on the desired final pH of the gel. when appropriate. The usual concentration of chitosan in the acetic acid is in the range of 1-6% (w/w). The chitosan powder mixture in acetic solution is usually stirred either or by the help of stirring aid. Chitosan hydrogels need to be allowed to swell for a sufficient time (24-48 hours). It is recommended to control the stirring speed to avoid trapping of air. Bath sonication can be used to remove entrapped air bubbles. Glycerol can be added in the initial mixture when required.